Coding

Part:BBa_K2481001:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


indB from Streptomyces chromofuscus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
    Illegal BamHI site found at 780
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons were optimized for expression in E. coli K12


Source

The DNA for this part was syntheisized by IDT, based on the Streptomyces chromofuscus coding sequences that was codon optimized for E. coli.

References